Douglas B. Craig
Biochemistry
Professor
B.Sc. (1988) Mount AllisonPh.D. (1993) Dalhousie
Post-Doc: Manitoba (1993-1994), Alberta (1994-1997)
phone: 204 786 9731
fax: 204 774 2401
email: d.craig@uwinnipeg.ca
Research Interests
Chemical studies typically involve measurements of large ensembles of
molecules with data representing averages. With respect to enzymes, we now
know such average values represent a simplification. Single enzyme
molecule assay utilizing ultrasensivitive capillary electrophoresis
laser-induced fluorescence instrumentation have demonstrated that
individual enzyme molecules are not identical. Individual molecules differ
with respect to rate and activation energy of catalysis. My research
interests are in the study of the basis and function of the heterogeneity
of enzyme molecules and its role within the cell. A second interest is the
development of ultrasensitive methods for the
analysis of biological molecules, particulary enzymes and proteins.
Lab photos
The Richardson College of the Environment and Sciences Building.
We moved into our new building during the summer of 2011.
This is my lab.
Same lab, different angle.
Top view of 1 of the 3 capillary electrophoresis instruments in my
lab.
A couple more images of the same instrument.
This instrument has a single detector.
Instruments use laser-induced fluorescence detection.
We have both red (633 nm) and green (543 nm)
helium-neon lasers,
and two solid state lasers (407 and 446 nm).
The other 2 instruments have 2 detectors.
This allows us to
detect two different fluorophores
independently at the same time.
We have started adding some basic molecular biology to our research.
We have equipment for PCR, running agarose gels, and various
incubators to let us clone and do a bit of mutagenesis.
...and of course what would a chemistry lab be
without a fume hood?
January 2015
Anna, Ankoor, Jeremie, Doug, Joanna
Killing Jeremie
Ankoor looks on approvingly
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